It can be used to quantitatively detect the content of anti-streptococcal hemolysin O in human serum or plasma in vitro.

Streptococcal hemolysin O. It is produced by group A, group C and group G of beta hemolytic streptococcus, and has strong antigenicity. Antibody ASO can be used as a marker of streptococcal infection. In general, the titer of Streptococcus began to rise one week after infection and reached its peak in 3-5 weeks. If there were no complications or re-infection, the titer declined two months later and dropped to the pre-infection level in 2-6 months. Positive results suggested that there had been hemolytic streptococcal infection recently, but it was not a marker of disease activity. It was used for assistant diagnosis of acute rheumatic fever and acute glomerulonephritis, and differential diagnosis of rheumatoid disease and rheumatoid arthritis.

ASO testing is based on latex immunoassay. When ASO is contained in serum, it reacts with SLO in R2 on latex particles, causing latex particles to agglutinate, thus forming turbidity. By measuring the turbidity generated by the reaction and comparing with the calibrated product, the concentration of ASO in the sample can be obtained.

1. Reagent preparation: liquid double reagent, no need to prepare direct use.

2. Test conditions: The test environment is not more than 95% at temperature (15-37)degrees Celsius and relative humidity.

Analytical sensitivity: When the activity was 75IU/L, the absorbance change value (A/min) was in the range of (0.01-0.1).

Linear range: (5-400) U/L

Accuracy: Relative deviation should be less than (+10%).

Precision: repeatability < 10%; batch difference < 15%.