The reagent can be used for quantitative detection of troponin I, myoglobin and creatine kinase isoenzyme in human serum, plasma or whole blood samples in vitro.

1. Take out the test card from the aluminum foil packaging bag and place it on a horizontal and dry surface.            

2. Insert the IC card into the fluorescence analyzer, click to read the IC card, confirm that the batch number of the IC card and the detection card match, and calibrate the IC card (see the instrument manual for details)           

3. Take 150 μ l whole blood sample, add 50 μ l sample buffer solution or 100 μ l plasma sample into the sampling hole of test card.           

4. After 15 minutes, insert the detection card into the card slot of the applicable instrument, and conduct quantitative interpretation of the results.

The contents of cardiac troponin I (cTnI), myoglobin (myo) and creatine kinase isoenzyme (CKMB) in human serum, plasma or whole blood samples were detected by fluorescence quantitative immunochromatography. When the sample is dropped into the sample hole of the detection card, cTnI, myo, CKMB in the sample combine with cTnI monoclonal antibody, myo monoclonal antibody and anti CKMB monoclonal antibody labeled with fluorescent substance in reagent pad to form reaction complex. The reaction complex moves forward along the nitrocellulose (NC) membrane with chromatography and is captured by the corresponding monoclonal antibody on the nitrocellulose (NC) membrane detection line, The amount of cTnI, myo and CKMB in the samples was positively correlated with the signal intensity of the detection area. The contents of cTnI, myo and CKMB in the samples were quantitatively detected by fluorescence analyzer.