The reagent can be used for quantitative determination of D-dimer in human plasma or whole blood samples in vitro.

1. Take out the test card from the aluminum foil packaging bag and place it on a horizontal and dry surface.           

 2. Insert the IC card into the fluorescence analyzer, click to read the IC card, confirm that the batch number of the IC card and the detection card match, and calibrate the IC card (see the instrument manual for details)            

3. Take 150 μ l whole blood sample, add 50 μ l sample buffer solution or 100 μ l plasma sample into the sampling hole of test card.            

4. After 15 minutes, insert the detection card into the card slot of the applicable instrument, and conduct quantitative interpretation of the results.

The content of D-dimer in human plasma or whole blood was detected by fluorescence quantitative immunochromatography. When the sample is dropped into the sample hole of the detection card, the D-dimer in the sample combines with the anti-D-dimer monoclonal antibody labeled with fluorescent substance in the reagent pad to form a reaction complex. The reaction complex moves forward along the nitrocellulose (NC) membrane along with the chromatography, and is captured by the corresponding monoclonal antibody on the nitrocellulose (NC) film detection line. The capture amount of D-dimer in the sample and the signal strength of the detection region are determined The content of D-dimer in the sample was detected by fluorescence analyzer.