The reagent can be used for quantitative determination of procalcitonin in human serum, plasma or whole blood samples in vitro.

1. Take out the test card from the aluminum foil packaging bag and place it on a horizontal and dry surface.           

 2. Insert the IC card into the fluorescence analyzer, click to read the IC card, confirm that the batch number of the IC card and the detection card match, and calibrate the IC card (see the instrument manual for details)            

3. Take 150 μ l whole blood sample, add 50 μ l sample buffer solution or 100 μ l plasma sample into the sampling hole of test card.           

 4. After 15 minutes, insert the detection card into the card slot of the applicable instrument, and conduct quantitative interpretation of the results.

The content of procalcitonin in human serum, plasma or whole blood was detected by fluorescence quantitative immunochromatography. When the sample is dropped into the sample adding hole of the test card, PCT in the sample The reaction complex was formed by combining with the anti PCT monoclonal antibody labeled with fluorescent substance in reagent pad. The reaction complex moved forward along the nitrocellulose (NC) membrane with chromatography and was captured by the corresponding monoclonal antibody on the detection line of nitrocellulose (NC) membrane. The amount of PCT captured in the sample was positively correlated with the signal intensity of the detection area. The content of PCT in the sample was quantitatively detected by fluorescence analyzer Quantity.