The reagent can be used for quantitative determination of N-terminal pro brain natriuretic peptide in human serum, plasma or whole blood samples in vitro.            

N-terminal pro brain natriuretic peptide is an important prognostic marker in patients with heart failure.

1. Take out the test card from the aluminum foil packaging bag and place it on a horizontal and dry surface.            

2. Insert the IC card into the fluorescence analyzer, click to read the IC card, confirm that the batch number of the IC card and the detection card match, and calibrate the IC card (see the instrument manual for details)            

3. Take 150 μ l whole blood sample, add 50 μ l sample buffer solution or 100 μ l plasma sample into the sampling hole of test card.            

4. After 15 minutes, insert the detection card into the card slot of the applicable instrument, and conduct quantitative interpretation of the results.

The content of N-terminal pro brain natriuretic peptide in human serum, plasma or whole blood was detected by fluorescence quantitative immunochromatography. When the sample is dropped into the sample hole of the detection card, the NT proBNP in the sample combines with the anti NT proBNP monoclonal antibody labeled with fluorescent substances in the reagent pad to form a reaction complex. The reaction complex moves forward along the nitrocellulose (NC) membrane along with the chromatography, and is captured by the corresponding monoclonal antibody on the nitrocellulose (NC) membrane detection line The content of NT proBNP in the sample was detected by fluorescence analyzer.